|
KMID : 0364219900330030330
|
|
Korean Journal of Zoology
1990 Volume.33 No. 3 p.330 ~ p.336
|
|
|
Studies on Esterase of Pieris rapae L. I. Changes of Esterase Activity and Zymogram Pattern During Development and Purification
|
|
Park Chul-Ho
Kim Hak-Ryul
Yoe Sung-Moon
|
|
|
Abstract
|
|
|
|
Changes in esterase activity and zymogram pattern during development in Pieirs rapae L. were investigated and three esterases (E2, E6 and E11) from the late 5th instar larvae were purified. Esterase activity in whole body increased rapidly during 5th instar larval stages and reached a peak at the late 5th instar larval stage. The number and intensity of esterase band from whole body and midgut also showed a peak at the late 5th instar larval stage. Purification of esterase was performed using gel filtration on Sephadex G-lOO, ion-exchange chromatography on DEAE-trisacryl and preparative electrophoresis. The final purities of these enzymes were about 30 to 60-fold.
|
|
|
KEYWORD
|
|
|
Cabbage worn , Exterase , Column chromatography
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|